Animal cells Sf9
Sf9 Insect cell growth and infection process by a baculovirus (BEVS)
The FOGALE BIOMASS SYSTEM signal provides
direct real time informations on:
>Cell growth monitoring:
The permittivity signal is correlated to viable cell count during the growth phase.
>Time of infection:
Cells are infected while they are in logarithm growth phase, the time of infection that is of great importance for productivity is accuretly determined.
>Success of baculovirus infection:
The plateau region in the permittivity profile (at 12 to 20h p.i.) has been related as an indicator of infection synchronicity (Zeiser et al, 2000)
>Harvest time:
It is of crucial importance for the yield and quality of the recombined protein.
The cell lysis monitoring helps in determining the optimal harvest time during the release phase.
Permittivity, biovolume in a batch culture of insect cell infected by a baculovirus.
Permittivity, viable cell count (trypan blue exclusion method), cell diameter (CASY counter)