Yeasts
Pichia pastoris growth and metabolism shift
The extremly high cell density-up to 100 g/l-in a highly aerated medium
offers an environment for evaluating the robustness of the FOGALE permittivity technique.
The FOGALE BIOMASS SYSTEM signal provides direct real informations on :
>Cell growth:
The permittivity signal is directly correlated to optical density (OD 600 nm)
>Time of induction phase (T1):
The permittivity profile allows us to identify the key events of the culture such as the metabolic shift required for heterologous protein
production and the applied methanol feed rate to avoid toxic metabolic accumulation (formaldehyde)
>Onset of stationnary phase (T2):
This phase detection is important since many secondary metabolites are formed and could be released, affecting the product quality.
Detection of the stationary phase and viability loss allows the decision to harvest the culture.
Permittivity and optical density in Pichia pastoris growth and heterologous protein production.